What is citrate utilization test?

Citrate utilization is a metabolic test used to determine an organism’s ability to utilize citrate as its lone source of carbon. Simmons citrate agar is a synthetic medium containing citrate, ammonium ions, and the pH indicator bromthymol blue.

What does a positive citrate utilization test look like?

At pH 7.5 or above, bromthymol blue turns royal blue. At a neutral pH, bromthymol blue is green, as evidenced by the uninoculated media. If the medium turns blue, the organism is citrate positive. If there is no color change, the organism is citrate negative.

Is the citrate utilization test selective or differential?

Simmons’ citrate agar is a selective and differential medium that tests for an organism’s ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. It is used for differentiating gram-negative bacteria on the basis of citrate utilization.

What is the principle of citrate utilization test?

Principle of Citrate Utilization Test Citrate is an organic acid, which hydrolysis will ultimately result in the production of alkaline carbonatesor bicarbonates. Simmons citrate agar is a media used to detect whether the test organism can exploit citrate or not. The citrate metabolism undergoes subsequent chemical reactions.

What is the definition of reconsideration in Iowa?

Definition of Reconsideration. Reconsideration means a review process that must be exhausted before an appeal hearing is granted. Such review processes include, but are not limited to, a reconsideration request through the Iowa Medicaid enterprise or its subcontractors, Magellan Behavioral Health Care, a health maintenance organization,…

What does colour change in citrate test mean?

The change in the pH of the solution gives details about the presence and absence of the test. Colour change of the solution to blue indicates the positive citrate test. If there is no colour shift, that means there is no growth in the medium, which indicates the absence of the citrate test.

What does reconsideration mean in the medical field?

Reconsideration means a written request to the payer or identified review organization by an injured employee or medical provider to reconsider a previous payer decision to deny medical treatment or services and that identifies the specific justification to support the request.