What is Bio Rad CFX96?

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.

What is PCR Bio Rad?

Polymerase chain reaction (PCR) is a technique used to exponentially amplify a specific target DNA sequence, allowing for the isolation, sequencing, or cloning of a single sequence among many. Typically, a PCR is a three-step reaction.

What is allelic discrimination?

Allelic Discrimination (AD) assay is a multiplexed, end-point assay that detects variants of a single nucleic acid sequence. The presence of two primer/probe pairs in each reaction allows genotyping of the two possible variants at the single-nucleic polymorphism (SNP) site in a target template sequence.

How do you read real time PCR results?

Real time PCR results are visualized in an amplification plot. Fluorescence is represented on the Y axis, whereas the number of PCR cycles is plotted in the X axis.

How much is a real time PCR machine?

A simple PCR machine like Bio-Rad T100 thermal cycler has a list price of 4912 USD (with a promotional price of 2595 USD in the US) as of Jan 30, 2019. The cost of rtPCR systems ranges anywhere from 15,000$ for some RotorGene models to over 90,000$ for QuantStudio 12k.

Does PCR have any limitations?

Although PCR is a valuable technique, it does have limitations. Therefore, PCR can only be used to identify the presence or absence of a known pathogen or gene. Another limitation is that the primers used for PCR can anneal non-specifically to sequences that are similar, but not completely identical to target DNA.

How do TaqMan probes work?

TaqMan probes consist of a fluorophore covalently attached to the 5′-end of the oligonucleotide probe and a quencher at the 3′-end. Degradation of the probe releases the fluorophore from it and breaks the proximity to the quencher, thus relieving the quenching effect and allowing fluorescence of the fluorophore.

How are SNPS alleles detected?

PCR-based methods for SNP/mutation detection are broadly categorized into two types-(1) polymorphic or mutant allele-directed specific analysis using primers matched with substituted nucleotide or using oligonucleotides to block or clamp the nontargeted template, and (2) melting curve analysis, which is combined with …

What is the purpose of real-time PCR?

Real-time polymerase chain reaction (real-time PCR) is commonly used to measure gene expression. It is more sensitive than microarrays in detecting small changes in expression but requires more input RNA and is less adaptable to high-throughput studies (1). It is best suited for studies of small subsets of genes.

What is quantitative real-time PCR used for?

Quantitative PCR (Q-PCR) was used to measure the amount of PCR product. It is the preferred method to measure quantitatively the levels of transgenic DNA. Q-PCR is often used to determine the number of copies in the sample.

Is the cfx96 real time PCR detection system interchangeable?

The CFX96 real-time PCR detection system builds on the power and flexibility of the C1000™thermal cycler, adding an easy-to-install interchangeable reaction module to create an exceptional real-time PCR system.

How many targets can a cfx96 system discriminate?

The CFX96 system can discriminate up to five targets in a single reaction well. The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels.

How are Bio-Rad plastic consumables validated for DNA?

Bio-Rad’s full line of plastic consumables has been validated to deliver reliable, reproducible results, leaving you one less thing to worry about. Each box of Bio-Rad tubes, plates, and caps is process-sampled and tested to be negative for DNase, RNase, and DNA. Successful Gene Expression

What are the benefits of the cfx96 system?

The CFX96 system’s temperature control produces high average ramp rates and tight uniformity during ramping, to yield fast time to target temperature and faster protocol run times. Run times can be dramatically shortened — to less than 30 minutes — while still producing accurate quantitative results.