How do you get rid of lipemia?

Conclusions: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples.

How do you run a lipemic sample?

A recommended procedure for treating lipemic samples is centrifugation using ultracentrifuge which effectively removes lipids and allows measurement of large number of analytes (42,43). However, due to the high cost, this equipment it is not available in a large number of laboratories.

How do you avoid lipemic samples?

One way to avoid grossly lipemic samples is to ask that patients fast for 12 hours before sample collection. If this is impractical, a mechanical-based means of clot detection should be available when samples are grossly lipemic.

What does Lipaemia mean?

: the presence of an excess of fats or lipids in the blood specifically : hypercholesterolemia.

What parameter is affected by lipemia?

Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.

How do you remove fat from your blood?

This article explores 13 ways to naturally reduce your blood triglycerides.

1. Aim for a healthy-for-you weight.
2. Limit your sugar intake.
3. Follow a lower carb diet.
4. Eat more fiber.
5. Exercise regularly.
6. Avoid trans fats.
7. Eat fatty fish twice weekly.
8. Increase your intake of unsaturated fats.

What does lipemic serum look like?

When the concentration of chylomicrons in the blood reaches a certain level the plasma which is normally light yellow in color will turn milky. This is called lipemia. At a high concentration of chylomicrons the plasma appears like milk with a tinge of pink. It is also turbid and opaque.

Is lipemia bad?

In the clinical laboratory setting, interferences can be a significant source of laboratory errors with potential to cause serious harm for the patient. After hemolysis, lipemia is the most frequent endogenous interference that can influence results of various laboratory methods by several mechanisms.

What is a lipemic serum?

Lipemia is defined as visible turbidity in serum or plasma samples due to the presence of lipoprotein particles, especially chylomicrons. The most common cause of turbidity is a high concentration of triglycerides [1,2]. It is the most common interference in outpatient populations, occurring in up to 7% of samples [3].

What causes too much fat in your blood?

Most people have high levels of fat in their blood because they eat too much high-fat food. Some people have high fat levels because they have an inherited disorder. High lipid levels may also be caused by medical conditions such as diabetes, hypothyroidism, alcoholism, kidney disease, liver disease and stress.

What causes Icteric blood sample?

Icteric serum is caused by the presence of excess bilirubin in the blood stream as a result of increased production (pre-hepatic) or inappropriate excretion (hepatic and post-hepatic).

Can lipemia affect test results?

Lipemia interferes with hematology tests by the following mechanism by light scattering. This affects the following results: Hemoglobin and hemoglobin-related indices: Results in falsely increased absorbance readings of hemoglobin, causing a falsely high measurement.

What kind of ultracentrifugation is used for lipemia?

I have been searching for a reference that supports the use of ultracentrifugation in lipemic samples for prothrombin time ( PT ), partial thromboplastin time ( PTT ), and fibrinogen ( FG ) tests. We are using a Siemens BCS with optical detection and have very good results with their 570 nm alternative for lipemic samples.

Which is the fastest microliter centrifuge in the world?

The MIKRO 200 / 200 R centrifuge ranks amongst the fastest microliter centrifuges in its class. Capable of holding 30 tubes per run at an RCF of up to 21,382 the MIKRO 200 offers high sample throughput and optimal separation results. Can be used to prepare samples in a wide variety of applications in molecular biology.

How long does it take to clean airfuge ultracentrifuge?

The Airfuge ® Air-Driven ultracentrifuge offers a simple, rapid method for removing chylomicrons (fat particles in lipemic samples) for accurate lipemic clarification results. Lipemic samples can be cleaned in 10 minutes, eliminating the need for time-consuming send-outs.

How to get lab results from lipemic specimens?

Take 10mL out and add 10mL of the patient, mix it up. And, I just take a little out of the sample because for whatever reason, our analyzer likes it to be half full. Now you can see a normal patient, our milky one and the 2 diluted specimens.

https://www.youtube.com/watch?v=-6wxXXsshmc