How do you make tetracycline stock solution?

Preparation

1. Step 1: To prepare 10 ml of 12 mg/ml tetracycline stock solution, weigh out 120 mg of tetracycline. Transfer it to a 15 ml screw-capped tube. Add 9 ml of 70% ethanol. Mix until all tetracycline dissolves completely. Tip:
2. Step 2: Adjust the volume to 10 ml with 70% ethanol. Mix it again.

How do you dissolve tetracycline?

Add 10 ml of sterile H2O (tetracycline hydrochloride has a different solubility than tetracycline). Dissolve completely.

What is aTc inducer?

Abstract. The transcriptional inducer anhydrotetracycline (aTc) and the bacteriostatic antibiotic tetracycline (Tc) are commonly used in all fields of biology for control of transcription or translation.

How do you dilute a stock antibiotic solution?

Preparation of 80ml stock solution

1. Tetracycline is kept in the 4C fridge in 68-564D.
2. Weigh 400mg of tetracyline HCL into a small weigh boat.
3. Dilute 95% Ethanol to 70% using milliQ water.
4. Add 80ml of 70% Ethanol to a 250ml bottle.
5. Add the tetracycline HCL to the ethanol.

How do you make a 1000X stock solution?

Preparation of 1000X Antibiotic Stock Solution

1. Weigh x grams of antibiotic-of-interest.
2. Dilute in pure 100% ethanol for chloramphenicol or sterile deionized water for kanamycin and ampicillin.
3. Syringe filter water-based antibiotic solution using a 0.22-μm filter.

What happens if you take too much tetracycline?

Nausea, vomiting, diarrhea, loss of appetite, mouth sores, black hairy tongue, sore throat, dizziness, headache, or rectal discomfort may occur. If any of these effects persist or worsen, tell your doctor or pharmacist promptly.

Does tetracycline dissolve in water?

Ethanol
AlcoholAlkali hydroxide
Tetracycline/Soluble in
This product is freely soluble in water, soluble in methanol and ethanol but insoluble in ether and hydrocarbons. (50 mg/ml) with heating, yielding a clear, yellow to yellow-orange solution. A clear, yellow green solution also results when 50 mg is dissolved in 4 ml 95% ethanol with heating.

What does Iptg bind to?

Like allolactose, IPTG binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner, thereby allowing the transcription of genes in the lac operon.

What is the purpose of the TetR gene?

TetR protein family members are mostly transcriptional repressors, meaning that they prevent the expression of certain genes at the DNA level. These proteins can act on genes with various functions including antibiotic resistance, biosynthesis and metabolism, bacterial pathogenesis, and response to cell stress.

How do you make Rifampinin stock solution?

Weigh 0.5 g of rifampicin [Rifampicin, GoldBio Catalog # R-120, (CAS 13292-46-1, MW = 822.94 g/mol)]. 2. Add 10 ml of 100% methanol. Dissolve completely.

How do you prepare a solution of stock?

Dilute the stock solution in distilled water to give working concentrations of 5, 10, 20, 30, 40, 50 mg/L. To prepare 50 ml of the working standards 5, 10, 20, 30, 40, 50 mg/L respectively, weigh 2.5, 5, 10, 15, 20 and 25 g of stock solution into 50 ml volumetric flasks and make up to the volume with distilled water.

How do you dilute a stock solution?

To make a dilution, you simply add a small quantity of a concentrated stock solution to an amount of pure solvent. The resulting solution contains the amount of solute originally taken from the stock solution but disperses that solute throughout a greater volume.

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