What are the components of Trypticase soy broth?

BD Tryptic Soy Broth Formula* Per Liter Purified Water Tryptone (Pancreatic Digest of Casein) 17.0 g Soytone (Peptic Digest of Soybean) 3.0 Glucose (= Dextrose) 2.5 Sodium Chloride 5.0 Dipotassium Phosphate 2.5 pH 7.3 ± 0.2 *Adjusted and/or supplemented as required to meet performance criteria.

Is trypticase soy agar selective or differential media?

ETSA agar is an enriched, non-selective, and differential medium. The nutritive base consists of casein, soy peptone, yeast extract, and dextrose.

What is trypticase soy agar used for?

Trypticase Soy Agar is used for the isolation and cultivation of fastidious as well as nonfastidious microorganisms, including anaerobic as well as aerobic bacteria, although it is not the medium of choice for anaerobes.

How do you make trypticase soy agar?

Preparation and Method of Use of Tryptic Soy Agar

1. Suspend 45 grams in 1000 ml distilled water.
2. Heat to boiling to dissolve the medium completely.
3. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.
4. Cool to 45-50°C.
5. Mix well and pour into sterile Petri plates.

What can you do with trypticase soy agar?

Trypticase soy agar. Trypticase soy agar or tryptone soya agar ( TSA) and Trypticase soy broth or tryptone soya broth ( TSB) with agar are growth media for the culturing of bacteria. They are general-purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow.

How big is a medium of trypticase soy broth?

Alternatively, You can use the commercially available Trypticase Soy Broth (TSB) media powders and simply weigh the mixture of content as prescribed on the carton or box of the manufacturer. As per HiMedia, its 30 grams for 1000 ml medium.

How are the components of trypticase can be modified?

The above-mentioned components can be modified by adding the various substances in a variety of ways as per the results required so that the rapid and satisfactory growth of the bacterial cell takes place.

How to prepare a TSB medium for trypticase?

REQUIREMENTS FOR THE PREPARATION OF TRYPTICASE SOY BROTH MEDIUM (TSB) 1 Sterile Conical Flask / Erlenmeyer Flask 2 Spatula 3 Pancreatic digest of casein 4 Papaic digest of soybean meal 5 Dextrose (Glucose) 6 Dipotassium hydrogen phosphate 7 Sodium Chloride 8 Measuring Cylinder 9 1N HCl 10 1N NaOH