What is the principle behind brine shrimp assay as a toxicity test?

Brine shrimp lethality bioassay is a simple, high throughput cytotoxicity test of bioactive chemicals. It is based on the killing ability of test compounds on a simple zoological organism-brine shrimp (Artemia salina). [1] This assay was first proposed by Michael et al., and further developed by several groups.

What is the purpose of brine shrimp lethality assay?

Brine shrimp lethality assay is an important tool for the preliminary cytotoxicity assay of plant extract and others based on the ability to kill a laboratory cultured larvae (nauplii). The nauplii were exposed to different concentrations of plant extract for 24 hours.

When should the hatched nauplii of brine shrimp collected for lethality assay?

Brine shrimp lethality test Approximately 12 h after hatching the phototropic nauplii were collected with a pipette from the lighted side and concentrated in a small vial.

Why are brine shrimp good animals to use in a toxicity experiment?

This is useful in the means of toxicity testing, because it is of high impor- tance to determine the concentration range in which there is a linear correlation between the concentration and the le- thality of the brine shrimps.

Why is the P > brine shrimp lethality assay important?

p>Brine shrimp lethality assay is an important tool for the preliminary cytotoxicity assay of plant extract and others based on the ability to kill a laboratory cultured larvae (nauplii).

What is the toxicity of brine shrimp nauplii?

In Brine Shrimp Lethality Bioassay, all the extracts produced dose dependent cytotoxicity effect to brine shrimp nauplii with methanol extract of leaf exhibiting highest toxicity having LC50 value 46.24 μg/ml where standard vincristine sulphate had the LC50 value of 0.69 μg/ml.

How is Annona reticulata used to kill brine shrimp?

Results showed that the extracts of Annona reticulata with Allium fistolisumand Brassica oleraceaewere potent against the brine shrimp when compared alone with combined extracts. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects.

How is in vivo lethality used in bioassay?

By this method, natural product extracts, fractions as well as the pure compounds can be tested for their bioactivity. The method utilizes in vivo lethality in a simple zoological organism (Brine nauplii) as a convenient monitor for screening and fractionation in the discovery of new bioactive natural products.