What is a species specific primer?
What is a species specific primer?
Among these techniques, species-specific primer PCR is a popular technology for species identification due to its high accuracy, sensitivity and convenience13. Both mtDNA (mitochondrial DNA) and rDNA (ribosomal DNA) are species-specific and can be used as molecular markers for species identification14,15.
What is the genus of Lactobacillus acidophilus?
Lactobacillus acidophilus (New Latin ‘acid-loving milk-bacillus’) is a species of gram positive bacteria in the genus Lactobacillus. L….
| Lactobacillus acidophilus | |
|---|---|
| Family: | Lactobacillaceae |
| Genus: | Lactobacillus |
| Species: | L. acidophilus |
| Binomial name |
How do you identify Lactobacillus species?
Lactobacillus isolates and several other strains of bacteria co-isolated on MRS medium from gastrointestinal ecosystem and fermented food products could be identified using DNA fingerprints generated by restriction endonucleases.
What contains Lactobacillus reuteri?
Lactobacillus reuteri is a probiotic culture and a member of the NSLAB population in several cheese varieties (e.g., Cheddar, Grana Padano, Gruyère, Idiazabal, Parmigiano Reggiano, Roncal, and Toma).
How are PCR primers used to identify lactobacilli?
One specific primer, LbLMA1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16S and 23S rRNA genes in a number of Lactobacillus strains. Amplification with LbLMA1-rev and R16-1, a universal primer, generated a PCR product for 23 Lactobacillus species.
Are there any genome based species specific primers?
Citation: You I, Kim EB (2020) Genome-based species-specific primers for rapid identification of six species of Lactobacillus acidophilus group using multiplex PCR. PLoS ONE 15 (3): e0230550. https://doi.org/10.1371/journal.pone.0230550
Are there species specific primers for L acidophilus?
In the present study, we investigated species-specific primer sets that were used to identify 6 species of L. acidophilus group ( L. gasseri, L. acidophilus, L. helveticus, L. jensenii, L. crispatus, L. gallinarum) via comparative genomics.
Where do I get my bacterial primer sets from?
All primer sets were synthesized from Bioneer (Daejeon, South Korea). To ensure that the individual primer sets were adequate for target species detection, 11 bacterial strains were used. Six bacterial strains were obtained from the Korean Agricultural Culture Collection (KACC), one from the Korean Collection for Type Cultures (KCTC).