What is the function of high performance liquid chromatography?

High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture.

What is meant by high performance liquid chromatography?

High-performance liquid chromatography (HPLC) is an analytical technique to separate, identify, and quantify components in a mixture. It is the single biggest chromatography technique essential to most laboratories worldwide.

What are the types of high performance liquid chromatography?

Different Types of HPLC Columns Used in Analysis

• Normal Phase HPLC Columns: This type of columns has more polar stationary phase than the mobile phase.
• Reverse Phase HPLC Columns:
• Ion Exchange HPLC Columns:
• Size Exclusion HPLC Columns:

Where does the high performance come from in high performance liquid chromatography HPLC )?

High performance liquid chromatography is basically a highly improved form of column chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres. That makes it much faster.

What are the disadvantages of liquid chromatography?

The main disadvantages of liquid chromatography (LC) compared to gas chromatography (GC) are as follows: LC has always been a slower technique than GC. Analysis times of GC of a few minutes are common. In contrast, LC traditionally has been a slower technique.

What is HPLC analysis?

HPLC analysis is one of the types of chromatography used to isolate and analyze mixtures. HPLC in full form is “High-pressure liquid chromatography.”. Unlike column chromatography, here high pressure is employed in the process. Hence the name.

What is the principle behind chromatography?

Principle of Chromatography. Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.

How does HPLC work?

HPLC has three steps: A small volume of your sample (in the liquid phase) is injected into your stationary phase. A pump moves the liquid down the column using high pressure. As the individual components exit the column, a detector measures them. This output is sent to a computer to produce a liquid chromatogram.