What is BCIP used for?

BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT.

How does NBT BCIP work?

BCIP/NBT Membrane Alkaline Phosphatase Substrate Description Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

How to prepare NBT solution?

NBT color development stock solution: Prepare 1 ml of 70% DMF solution by mixing 0.7 ml of DMF with 0.3 ml of dd water. Dissolve 30 mg of NBT in this 70% DMF solution. C. BCIP color development stock solution: Dissolve 15 mg of BCIP in 1 ml of DMF.

What is the purpose of adding BCIP NBT?

BCIP (1) is the alkaline phosphatase (AP) substrate, which after dephosphorylation is oxidized by NBT to yield a dark-blue indigo pre- cipitating dye. NBT (2) is thereby reduced to a dark-blue precipitating dye and serves to intensify the color reaction making the detection more sensitive.

Which is Thermo Scientific 1 step NBT / BCIP solution?

Final detection was with Thermo Scientific 1-Step NBT/BCIP Substrate Solution. 5-bromo-4-chloro-3-indolyl phosphate (BCIP) has a molecular weight of 433.6, and hydrolysis by alkaline phosphatase (AP) results in a blue-purple precipitate that can be deposited on nitrocellulose or nylon membranes.

What do you need to know about BCI integrated solutions?

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Who are the in-house sales engineers for BCI?

Our in-house sales engineers will assist you in every step of the process for designing code-compliant solutions. BCI provides a wide range of solutions to protect your assets with mass notifications, distributed antenna networking, public safety systems, and several other life safety systems.

When to use Pierce NBT and BCIP powders?

Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity). For Research Use Only. Not for use in diagnostic procedures. Upon receipt store product at 4°C. Product is shipped at ambient temperature.