Can MACS columns be reused?

The MACS columns fit perfectly on the stopcocks where the miniPREPS are supposed to go. Once columns are used, transfer to qiavac manifold stopcocks, and add ddH2O per column, 3X, with a squirt bottle. Then, add ETOH 3x using another squirt bottle, always for all the alcohol to clear the column between refill.

What are LS columns?

LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells. LS Columns can be used with the following separators: MidiMACS™ Separator.

What is MS column?

MS Columns are designed for positive selection of cells. They are also suitable for depletion of strongly magnetically labeled cells.

What does Macs stand for Miltenyi?

Magnetic-activated cell sorting
Magnetic-activated cell sorting (MACS) is a method for separation of various cell populations depending on their surface antigens (CD molecules) invented by Miltenyi Biotec.

How are Miltenyi nanobeads removed from T cells?

Miltenyi nanoparticles accumulating in human CD3+ T cell vesicles. After 72 hours (see new TEM’s below), Miltenyi nanoparticles are not biodegraded. In contrast, Dynabeads are released and removed from the cells. Notes: All video imagery is actual footage and unaltered. In Germany, this webpage is not allowed for viewing.

How many countries does Miltenyi Biotec sell in?

In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries. For exact search enclose your search term with “quotation marks”.

How does Miltenyi microbeads interact with your T cells?

CD3 Microbeads (Cat. No. 130-050-101, Miltenyi Biotec, Germany) were labeled with highly cross absorbed goat anti-mouse IgG (H+L) Alexa 555 ( Cat. No. A21424 ). The staining of CD3 MicroBeads was specific as adding the anti-mouse IgG Alexa 555 antibody directly to PBMC did not create a signal in a fluorescent microscope.

What kind of dye is used for Miltenyi microbeads?

In some experiments, LysoTracker Green Dye ( Cat. No. L7526) was added during the incubation. CD3 Microbeads (Cat. No. 130-050-101, Miltenyi Biotec, Germany) were labeled with highly cross absorbed goat anti-mouse IgG (H+L) Alexa 555 ( Cat. No. A21424 ).