What does RNase 3 do?

RNase III enzymes fulfill important functions in the processing of the precursors of various stable noncoding RNAs such as ribosomal RNAs and small nuclear and nucleolar RNAs. In addition, they cleave and promote the degradation of specific mRNAs or improperly processed forms of certain mRNAs.

What class of enzyme is RNase A?

ribonucleases
RNases, or ribonucleases, are a broad class of RNA-degrading enzyme possessed by all organisms, and are extremely common in the environment. Because RNase is specific to cleaving RNA molecules, the enzyme is commonly used in molecular biology for removal of RNA within DNA and protein samples.

What is RNase used for?

RNase A treatment is used for the removal of RNA from genomic DNA samples. RNase A cleaves the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphate group attached to the 3′- ribose of an adjacent pyrimidine nucleotide.

What is Dicer in genetics?

Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. Dicer facilitates the activation of the RNA-induced silencing complex (RISC), which is essential for RNA interference.

How does RNase III work in E coli?

In E. coli, RNase III cleaves ribosomal RNA (rRNA) precursors during maturation of rRNA. The enzyme cleaves dsRNA into 12–15 bp dsRNA fragments with 2 to 3 nucleotide 3′ overhangs, and 5′ phosphate and 3′ hydroxyl termini.

How does RNase III regulate the expression of genes?

We examine here how RNase III itself is regulated in response to growth and other environmental changes encountered by the cell and how, by binding or processing double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes.

What kind of RNA is shortcut RNase III?

ShortCut® RNase III. ShortCut RNase III converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA). Isolated from a recombinant source.

What are the termini of Ambion RNase III?

The enzyme cleaves dsRNA into 12–15 bp dsRNA fragments with 2 to 3 nucleotide 3′ overhangs, and 5′ phosphate and 3′ hydroxyl termini. The termini and overhangs of RNase III cleavage products are thus the same as those produced by Dicer in the eukaryotic RNAi pathway.