What is the principle behind gel filtration chromatography?

Principle. The gel filtration chromatography is based on the molecular size and the hydrodynamic volume of the components. Smaller molecules get distributed between the mobile phase and the outside of the sieve. Then, they pass through the column at a slower rate and appear later in the effluent.

How can gel filtration chromatography be improved?

Increase in column length increases the resolution and increase in column diameter results in high bed volume and hence higher column capacity. The fractionation range and the exclusion limit can be controlled by varying pore size. The smaller the particle size of the gel, the higher the resolution achieved.

What is gel permeation chromatography used for?

Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size, typically in organic solvents. The technique is often used for the analysis of polymers.

What is a gel filtration column?

Medical Definition of gel filtration. : chromatography in which the material to be fractionated separates primarily according to molecular size as it moves through a column of a gel and is washed with a solvent so that the fractions appear successively at the end of the column.

What is gel chromatography?

Gel chromatography. Written By: Gel chromatography, also called Gel Filtration, in analytical chemistry, technique for separating chemical substances by exploiting the differences in the rates at which they pass through a bed of a porous, semisolid substance.

What is molecular exclusion chromatography?

Molecular exclusion (molecular sieve) chromatography is based on the difference in permeability of the component molecules in the stationary phase (a highly porous, nonionic gel). It may be subdivided into gel permeation chromatography (GPC), in which the eluent is a nonaqueous solvent, and gel filtration,…