How is the Ames test performed?

The Ames Test combines a bacterial revertant mutation assay with a simulation of mammalian metabolism to produce a highly sensitive test for mutagenic chemicals in the environment. A rat liver homogenate is prepared to produce a metabolically active extract (S9).

Which microorganism is used in Ames test?

Ames test devised by a scientist “Bruce Ames” is used to assess the potential carcinogenic effect of chemicals by using the bacterial strain Salmonella typhimurium. This strain is mutant for the biosynthesis of histidine amino acid.

Are Auxotrophs used in Ames test?

The Ames test utilizes specific strains of the bacteria Salmonella typhimurium as tools to detect mutations. These strains of S. typhimurium used are known as auxotrophs.

What can you do with xenometrix Ames test?

Xenometrix offers ready-to-use kits, individual bacterial and yeast strains, rat liver S9, reagents and service analytics optimized for studies in the fields of mutagenicity, genotoxicity, endocrine disruption or skin permeation.

What is the principle of the Ames test?

Principle Ames test uses several strains of bacteria (Salmonella, E.coli) that carry a particular mutation. Point mutations are made in the histidine (Salmonella typhimurium) or the tryptophan (Escherichia coli) operon, rendering the bacteria incapable of producing the corresponding amino acid.

What kind of mutations are used in the Ames test?

Ames test uses several strains of bacteria ( Salmonella, E.coli) that carry a particular mutation. Point mutations are made in the histidine (Salmonella typhimurium) or the tryptophan (Escherichia coli) operon, rendering the bacteria incapable of producing the corresponding amino acid.

What kind of bacteria can the Ames test detect?

Ames test can detects suitable mutants in large population of bacteria with high sensitivity. Ames assay consists of Salmonella typhimurium strains and so it is not a perfect model for human.