How do you interpret MALDI?

Interpreting a MALDI spectrum One is to regard it de novo, and to try to deduce, without other input, what the peptides might be. The other method, is to use a library of protein sequences, and to try to find proteins in the library which could have given rise to the peaks in the spectrum.

What does MALDI-TOF measure?

Mass spectrometry is an analytical technique in which samples are ionized into charged molecules and ratio of their mass-to-charge (m/z) can be measured.

What is a MALDI-TOF assay?

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) is a powerful analytical mass spectrometry technique that has generated numerous diagnostic and clinical applications, especially for the identification of microorganisms for medical diagnosis.

What can MALDI TOF mass spectrometry be used for?

MALDI-TOF is extremely versatile and can analyze a broad range of samples, which has led to numerous applications in basic research and clinical diagnostics.

What is the working principle of MALDI TOF?

Working Principle of MALDI-TOF Mass Spectrometry . The MALDI TOF process is a two-phase procedure; Ionization Phase; Time of Flight Phase; Ionization Phase: Initially, the samples are fixed in a crystalline matrix in a target plate and are bombarded by a laser. The sample molecules vaporize into the vacuum while being ionized at the same time.

How are Frag-ments considered in the MALDI spectra?

As MALDI was just introduced mainly ions formed intactly were considered and some frag- ments were considered only as an exception. On evaluating the mass spectra, first only those additional signals corresponding to fragment ions were recognized, which were formed during the desorption process inside the source (in-source decay, ISD).

What does TOF stand for in mass spectrometry?

TOF stands for Time of Flight, a mass spectrometry method that separates ions by their mass to charge ratio and determines that mass to charge ratio by the time it takes for the ions to reach a detector.

https://www.youtube.com/watch?v=Z9MRg9CrQcE