What passes through a cation exchange column?

Thus, in cation exchange column, the chemical groups attached to the beads are negatively charged groups and will have positively charged counter-ions and positively charged compounds present in a mixture passed through the column will exchange with the counter-ions and “stick” to the negatively charged groups on the …

How does a cation exchange column work?

Ion exchange chromatography separates ions and molecules based on their net overall surface charge. The media in a cation exchange column is negatively charged, binding positively charged molecules, and therefore cations are used for elution of the bound molecules.

What happens in cation exchange chromatography?

Cation exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Cation exchange chromatography, more specifically, uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.

How are cations used in ion exchange chromatography?

Ion exchange chromatography separates ions and molecules based on their net overall surface charge. The media in a cation exchange column is negatively charged, binding positively charged molecules, and therefore cations are used for elution of the bound molecules.

Which is better a weak or strong cation exchange column?

Weak cation exchange columns have a narrower pH operating range than strong cation exchange columns. However, working at this narrow pH range is generally less denaturing for most proteins.

How do you change a cation exchange column?

1) Close the inlet valve (connected to the top of the column) or, in the case if a duty/standby column arrangement, switch the inlet and outlet valves to the standby column. 2) Remove the quick-release coupling from the top and bottom of the spent column. 3) Mark the top of the column and pull it from the mounting clips.

How does the pH affect the charge of a cation exchange column?

When using a strong cation exchanger, the pH of the buffer (mobile phase) has little impact on the charge of the column but does affect the charges of the components in the sample.