What is the pH of a Bio-Gel P gel?
What is the pH of a Bio-Gel P gel?
Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C. The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents.
What kind of Bio-Gel is used for column packing?
Bio-Gel P2 (Bio-Rad, USA) with particle diameter 200 mesh was used for column packing . The peptides for trial separations (latrotoxin fragment and immunofan) were synthesised in our laboratory, and 95% purity was confirmed by HPLC and amino acid analysis.
How are Bio-Gel P polyacrylamide gels prepared?
Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N, N’ -methylenebisacrylamide. Bio-Gel P gels: Are supplied dry and are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
What is the molecular weight limit for Bio-Gel p-6dg?
Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilic nature ensures little or no interaction between the gel and sample molecules.
Can you use Bio-Gel P gel with Dis tilled water?
Bio-Gel P gel may be used effectively with dis- tilled water however, buffers of > 50 mM ionic strength are recom- mended for most protein separations. Miscible organic solvents may be added to the eluants used with Bio-Gel P gel.
When does the P100 particulate filter come out?
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