How do you increase stomatal density?

The density of stomata on a leaf is recorded per unit area, usually the number per sq mm.

1. A microscope is calibrated so that its field of view is known.
2. Its area can be calculated using formula.
3. Where.
4. r = radius of the field of view.
5. The area of the field of view is 0.13 mm 2.

What is used to see stomata clearly?

Viewing Stomata with a Microscope In order to examine the stomata of a plant leaf, paint clear nail polish on the leaf, avoiding the veins if possible. You can paint both the top and the bottom of the leaf.

How can we demonstrate the presence of stomata in a leaf?

Procedure

1. Pick a healthy leaf from the potted plant.
2. Fold the leaf to gently pull the peel apart to separate a peeled section from the lower surface of the leaf.
3. In the watch glass, stain the sample by adding some drops of safranin through a dropper.
4. Take the peel out after 2-3 minutes.

How to get an impression of a leaf stomata?

Place a piece of clear tape over the dried nail polish.   Gently but firmly press the tape onto the leaf. 4. Peel the tape from the leaf and place the tape sticky side down onto a microscope slide. Examine the tape, which now contains an impression of the leaf cells. Try to use both scanning and low power. 5.

What kind of paint to use on leaf stomata?

On the underside of the leaf (bottom/non-shiny side) paint on a couple of strokes of clear nail polish and leave to dry.

How to investigate the distribution of stomata and guard cells?

Put a small drop of water on a microscope slide. Hold the leaf with the surface you want to examine uppermost. Tear the leaf at an angle so as to reveal part of the epidermis. Place the leaf on the microscope slide and examine. Paint the surface of the leaf with clear nail varnish. Allow to dry. Peel off the nail varnish with forceps.

What’s the best way to view stomata under the microscope?

Allow the nail polish to dry completely, and then place a piece of CLEAR cellophane tape over the nail polish. Matte tape won’t work. Also, students need to make sure they aren’t leaving fingerprints on the tape before use. Using their fingernail or school ID, rub the tape down firmly over the nail polish. Slowly peel the tape off of the leaf.