What are the different types of PCR techniques?

Some of the common types of PCR are;

• Real-Time PCR (quantitative PCR or qPCR)
• Reverse-Transcriptase (RT-PCR)
• Multiplex PCR.
• Nested PCR.
• High Fidelity PCR.
• Fast PCR.
• Hot Start PCR.
• GC-Rich PCR.

What is another way PCR can be applied?

Other applications of PCR include DNA sequencing to determine unknown PCR-amplified sequences in which one of the amplification primers may be used in Sanger sequencing, isolation of a DNA sequence to expedite recombinant DNA technologies involving the insertion of a DNA sequence into a plasmid, phage, or cosmid ( …

What technique is used for detection of PCR product?

agarose gel electrophoresis
The most widely used method for analyzing the PCR product is the use of agarose gel electrophoresis, which separates DNA products on the basis of size and charge. Agarose gel electrophoresis is the easiest method of visualizing and analyzing the PCR product.

What kind of machine is used for PCR?

Quantitative PCR (qPCR), also called real-time PCR, or RT-PCR, is a variation of the standard polymerase chain reaction which uses just one machine to combine the amplification of a target DNA sequence with the quantification of the concentration of the DNA in any given reaction. This is done using fluorescence-detecting thermocyclers.

What are the different types of PCR amplification?

1 Loop-mediated isothermal amplification (LAMP) 2 Rolling Circle Amplification (RCA) 3 Helicase-Dependent DNA Amplification (HDA)

How are thermocycling and multiplex PCR techniques used?

Thermocycling techniques use temperature cycling to drive repeated cycles of DNA synthesis. Multiplex PCR is a type of PCR technique which allows an amplification of many target sequences concurrently in the same reaction mixture. A single reaction mixture includes sets of primer pairs for different DNA targets.

How are PCR methods used in biological research?

Many other PCR methods are well utilized in biological research. Colony PCR can be used to screen for the presence of a specific genomic insert from bacterial colonies without the need for culturing or plasmid purification [ 32 ]. Genotyping often uses allele-specific PCR [ 33 ].